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- targeted gene walking PCR 靶基因步移PCR
- Analyse the genotype of the target genes using PCR. 聚合酶链反应(PCR)扩增目的基因进行基因型分析。
- RESULTS: The target gene of recombinant adenovirus plasmid could be amplified by PCR in different passages of HEK293 cells. 用减蛋综合征阳性血清在体外中和重组腺病毒,接种HEK293细胞;
- Methods: The 681 bp target gene was amplified from heart tissue of young rats with RT PCR, fusion gene of huIL 2/myosin was constructed by splicing with huIL 2 cDNA using ligation methods and its RV was constructed. 方法 :以 RT- PCR方法从幼龄大鼠心肌组织中扩增出 6 81bp的目的基因 ,与 hu IL - 2 c DNA序列拼接 ,构建融合基因 hu IL - 2 / m yosin及其逆转录病毒载体。
- Methods Blood samples were collected from a Uyghur population in Xinjiang Uyghur Autonomous Region,and the genomic DNA was extracted from the leucocytes and the target gene fragment amplified by PCR. 方法收集新疆维吾尔族自治区维吾尔族一般人群血标本,提取白细胞基因组DNA,以PCR扩增目的基因片段并应用荧光探针杂交可视技术检测点突变。
- Liver Stem Cells as Vector for Targeted Gene Therapy of Hepatocellular Carcinoma. 肝干细胞作为肝细胞癌靶向基因治疗载体的实验研究
- Targeted gene replacement (TGR) is an important technique for gene function analysis. 目标基因替换是基因功能分析的重要手段。
- AIM: To clone human soluble TRAIL cDNA and construct its eukaryotic expression vector to prepare a condition for targeted gene therapy. 克隆人可溶性trail(strail)基因并构建肿瘤细胞特异性真核表达载体;为肿瘤基因治疗的靶向性奠定实验基础.
- The target gene C-erbB-2 and control gene thymidine kinase are located on chromosome 17. The different size of two PCR fragments were produced by simulataneously performing PCR on test and control genes in the same PCR mix. 靶基因C-erbB-2和对照基因thymidine kinase均定位于17号染色体。 在同一反应体系中进行PCR反应可产生两个大小不同的PCR扩增的特异性片段,然后通过2%25琼脂糖凝胶电泳将两片段分开,再用密度测定仪测定靶基因与对照基因的密度比率,当两者密度比率大于1时,表明存在C-erbB-2原癌基因扩增。
- Molecular marker-assisted selection (MAS) provides a very efficient tool for backcross breeding because it can offer rapid and precise selection of the targeted gene. 分子标记辅助选择技术以其对目标基因快速而精确的选择为回交育种提供了非常有效的工具。
- The silencing of target gene transcription by unliganded NRs(including AR) may be mediated by these corepressors. 非配体化的核受体(NRs)抑制靶基因的转录可能是通过这些辅阻遏子来介导的。
- Method The virulence genes of Shiga like toxin(SLT 1 and SLT 2), intimin(eaeA) and hemolysin(hlyA) were chosen as the target genes and amplified in multiplex PCR assays. Results Of the eighty five E. 方法 应用肠出血性大肠杆菌 (EHEC)的多重引物聚合酶链反应 (PCR)方法 ,以志贺样毒素 (SLT2 和SLT1)基因、“粘附抹平”因子eaeA基因和溶血素 (hly)基因为靶基因进行检测。
- Detection of target gene expression in paraffin-embedded tissues is feasible by RT-PCR or RQ-PCR. 应用RT-PCR或RQ-PCR方法在石蜡包埋组织中检测目的基因的表达是可行的。
- The basic principle, characteristic and its application of PCR in the preparation of target genes, medicine, forensic medicine, paleontology and ecology research are introduced in this paper. 本文综述了PCR技术的基本原理、特点及在目的基因制备、医学、法医学、古生物学及生态学中的应用。
- The target gene was then inserted into expression vector pET-28a and expressed in E.coli BL21 with IPTG induction. 将mdlA基因克隆到原核表达载体pET-28a中,转化大肠杆菌BL21(DE3),经诱导后,重组的脂肪酶在宿主菌中的表达量可达菌体总蛋白量的45.;2%25。
- Cloning of S-locus gene by PCR walking in Brassica campestris L. PCR步行法克隆油菜自交不亲和基因。
- Conclusion: The prokaryotic expression vector with target gene was constructed successfully. 结论:成功构建了带有目的基因的原核表达载体。
- The therapy of antiangiogenesis and target gene offer a broad clinic prospect for lung cancer. 内皮抑素的抗血管生成治疗和血管靶向基因治疗,为治疗肺癌提供了广阔前景。
- Target gene combined with proper vector is transformed in Ecoli cells to amplify. 用适当的载体与目的分子连接后,转入大肠肝菌宿主细胞扩增。
- It was reported in the past decade that a targeted gene inactivation of some transcription factors could specifically affect the development of some groups of neurons in the brainstem and then resulted in distinct respiratory phenotypes. 近年来,人们利用分子生物学技术研究发现,小鼠某些基因突变可影响呼吸中枢特定神经元的发育,并由此导致特殊的呼吸表型。