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- Methods The DNA fregment target to the translation initiation site of CCR5 mRNA obtained by RT - PCR from peripheral blood mononuclear cells(PBMCs)was cloned into retro-viral vector pLXSN. 方法 用RT-PCR从健康人外周血单个核细胞(PBMCs)中获得趋化因子受体CCR5翻译起始区的基基片段,用基因重组技术将此目的基因以正、反两个方向定向插入到逆转录病毒载体pLXSN。
- Methods The ASODNs including the translation initiation site of ATP7B mRNA was artificially synthesized, and the sense oligodeoxynucleotide corresponding to the ASODNs was also synthesized as control. 方法 人工合成包含ATP7B mRNA 翻译起始位点的反义寡核苷酸片段及正义寡核苷酸片段作为对照。
- Based on the position weight matrix(PWM) and length distribution of open reading frame(ORF),a simple method for predicting translation initiation sites is presented. It can identify TIS from upstream AUGs easily. 结合位置权重矩阵(PWM;position weight matrix)和开放阅读框架(ORF;open reading frame)的长度分布特征建立了简单的方法识别翻译起始位点;此方法能很好地区分上游AUG和TIS.
- Using Ribosome Scanning Model to Predict Translation Initiation Sites 用核糖体扫描模型预测翻译起始位点
- translation initiation site 翻译起始位点
- AUG depletion may be an indication that upstream AUG can probably disturb the translation initiation of bacteria and archaea. AUG的缺失表明在起始密码子上游的AUG很可能会对基因的翻译起始产生影响。
- The primer extension assay found the putative transcription initiation site near the position of DHSS. 引物延伸实验结果显示主要的转录起始位点的位置与 DNase1高敏位点的位置具有一致性 ,与瞬时表达实验结果也具有相关性。
- The sequences and secondary structures of IRES are compared.Interaction between IRES and translation initiation factors and relation with virulence of FMDV is summarized. 本文对IRES的一、二级结构进行了比较,对IRES与翻译起始因子的相互作用以及对病毒毒力的影响作了综述。
- A vitamin D receptor gene polymorphism in the translation initiation codon: effect on prote in activity and relation to bone mineral density in Japanese women. 更难理解的是为什么这些等位基因有时存在,有时不存在,有时甚至是相反的结果。
- Treatment with two oligonucleotides directed against the coding region and the translation initiation region of bcl 2 messenger RNA/VP 16 combination respectively for 48 h had significantly reduced the number of viable AL cells. 靶向 bcl- 2 m RNA翻译起始区与靶向蛋白编码区的两个反义寡核苷酸分别与 VP1 6 联合作用 AL 细胞 4 8h;细胞的生存受到明显的抑制 ;分别同无关寡核苷酸 (NS- ODN)联合 VP1 6 组、单用 VP1 6 组进行比较 ;差异有显著性 (P<0 .;0 5 )。
- Objective To investigate the effect of a new cloned full length gene, C2 gene which encodes a translation initiation factor, on cell cycle and the relationship between C2gene and apoptosis. 目的探索新克隆的编码人蛋白翻译起始因子的全长基因(C2基因)对细胞周期的影响及与细胞凋亡的关系。
- There is a secondary struct ure complex at the translation initiation region of apr mRNA.When one of the se condary structure complex at the translation initiation region of apr mRNA. 依赖链霉素突变在翻译层次抑制碱性蛋白酶基因的表达,但对中性蛋白酶基因的表达没有影响。
- For the notched specimens, there are multiple crack initiation sites on the fractography. 瞬断区所承受的断裂应力随循环应力升高而降低;
- The initial site of recurrence was determined in 28 patients (loco regional in 10 and distant in 18). 28个病人取决于开始复发的位置(其中10例局部侵犯、18例远处转移)。
- The result shows that the fracture has appeared fatigue feature, with initiation sites at the root grooves of the spline of the shafts. 结果表明,轴的断口具有明显的疲劳特征,疲劳源位于二轴花键槽根部。
- TFBS is usually 5 to 20 bp in length and situated variously relative to the transcription initial site (TIS) of a given gene. 转录因子结合位点的长度通常为5到20bp,相对于基因的转录起始位点(transcription initial site,TIS)有多种位置。
- Factors in the Translation Initiation of FMDV 影响口蹄疫病毒转译起始的因素
- A STUDY ON PITTING INITIATION SITE OF CARBON STEELS 低碳钢点蚀诱发部位的实验研究
- Eukaryotic Translation Initiation Factors and Tumor 真核翻译起始因子与肿瘤
- Site plans will be further fleshed out, with a view to optimizing both use and after-use.Demolition, relocation and other aspects of initial site preparation will be carried out. 坚持办展与后续利用相结合的原则,继续深化世博园区实施规划,推动世博园区动拆迁等前期开发工作,加快推进世博会重大项目建设。