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- Low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture. 方法:采用低浓度、短时间胰酶消化和机械分离相结合的方法制备皮层神经元悬液,进行培养。
- Methods: Low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture. 方法:采用低浓度、短时间胰酶消化和机械分离相结合的方法制备皮层神经元悬液,进行培养。
- The whole spinal cords of neonatal rats were dissociated and single cells were separated by trypsin digestion. 本研究探索利用新生大鼠脊髓培养和纯化少突胶质细胞的方法。
- N-and C-half molecules containing a single iron-binding site were simultaneously obtained from trypsin digest of iron-saturated pig transferrin. 摘要用胰蛋白酶水解铁饱和的猪血清转铁蛋白,可同时获得含单一铁结合部位的N端和C端半分子。
- Trypsin digestion was used in the subculture of mRTECs.The types of cells were identified by cellular immunocytochemistry. 胰蛋白酶消化传代,以免疫细胞化学方法鉴定细胞种类。
- Clear and abundant G-banding at late prophase,prometaphase and early metaphase along the length of the tea chromosome has been induced by trypsin digestion. 摘要 本文应用胰酶法在茶树的晚前期、前中期和早中期的每一染色体的全长上诱导出了清晰而丰富的G带带纹。
- Conclusion Collagenase and trypsin digestion assisted by immuno-magentical column isolation is effective for isolation of type-A and type-B synoviocytes. 结论胶原酶和胰蛋白酶联合消化,免疫磁柱法是一种确切有效的分离人类关节滑膜A、B型细胞的方法。
- The embryonic lung cells were isolated by trypsin digestion of a human embryonic lung obtained from a therapeutic abortion case and was designated as cell line Elu 8801. Anopheles stephensi mosquitoes were infected by biting P. b. 用胰酶消化法自人工流产胎儿分离出胚肺细胞后,建立Elu 8801细胞株。
- Methods Cardiomyocytes and cardiac fibroblasts were isolated by trypsin digestion method. DNA and protein synthesis were measured by 3 H-deoxythy-midine( 3 H-TdR) and 3 H-Leucine( 3 H-Leu) incorporation. Protein content was measured by Bradford method. 方法采用体外乳鼠心肌细胞和心脏成纤维细胞培养,一定浓度的ET-1、ANP单独或联合作用细胞24h,以3H-胸腺嘧啶脱氧核苷(3H-TdR)、3H-亮氨酸(3H-Leu)掺入法测定细胞DNA、蛋白合成,Brad-ford法测细胞总蛋白含量,RT-PCR法测心肌细胞心钠素ANPmRNA的表达,评价ET-1、ANP对心肌细胞和成纤维细胞的作用。
- In the present study, the hepatocytes were obtained through shearing 9-day-old Sanhuang chicken embryo liver tissue, separating by trypsin digestion and culturing in revised M199 culture. 摘要剪切9日龄三黄肉鸡鸡胚肝组织,胰酶消化分离肝细胞,用改良的M199培养液进行培养。
- At last, on-target tryptic digestion and protein molecular weight detection are adopted to identify phosphoproteins. 最后蛋白检测采用蛋白分子量检测和靶上酶解来鉴定蛋白,这样的分析方法快速、简单和可靠。
- We have compaired two methods which were explant culture and culture of SC with rapid trypsinization digestion primarily. 我们采用组织块培养法和酶消化法培养及用Ara-c纯化SC,观察SC生长情况并进行了初步的比较。
- This research demonstrated that fish gelatin can be differentiated by detection of marker peptides in the tryptic digest of sample gelatin. 结果表明利用液相色谱-质谱联用技术通过检测酶解产物中的特征多肽是鉴别鱼明胶的有效方法。
- This research demonstrates that it is possible to determine the relative content of different collagens with HPLC/MS coupled with thermal denaturation and tryptic digestion. 本课题研究结果表明使用高效液相色谱/质谱联用技术分析组织中的胶原蛋白类型及其动态变化具有可行性,为更好的临床应用提供了实验基础。
- It took me some time to digest what I had heard. 我花了一些时间才把听到的东西弄明白。
- This rich food doesn't digest easily. 这种油腻的食物不易消化。
- Saliva helps one chew and digest food. 唾液能帮助咀嚼和消化食物。
- Reader's Digest is a periodical. 《读者文摘》是一种期刊。
- A comprehensive digest or complete treatise. 总论,纲要详细的摘要或整个论文
- Gastric juices help us digest our food. 胃液能帮助消化。