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- 利用PCR方法以人胎肝cDNA文库为模板,克隆重组人肝细胞生长因子(rhHGF)全长cDNA,经序列分析后,插入pPIC9K中,构建多拷贝串联rhHGF基因酵母分泌型表达载体pPIC9K-rhHGF,转化酵母宿主菌Pichia Pastoris GS115。The cDNA encoding rhHGF was amplified by PCR using cDNA library of fetal liver tissues as template,and was insert-ed,after being confirmed by DNA sequence analysis, into the vector pPIC9K to form a recombinant Pichia pastoris secretory expression vector pPIC9K-rhHGF ,which contain many copies of rhHGF gene .
- 构词因素
- pPIC9K表达载体Pichia pastoris
- 表达to voice (an opinion)
- 表达载体pPIC9KExpression vector pPIC9K
- 构建了真核表达载体pPic9k-DCN，并将酶切线性化的重组载体转入酵母菌HIS~-/GS115后，筛选出了抗高浓度(4mg/ml)G418的阳性克隆，并用PCR法进行了鉴定。We have made up an eukaryotic (Pichia pastoris) expressive vector (pPic9k-DCN). After the linear recombinant vector was introduced into HIS~-/GS115 cells,we have selected positice clones against G418(4mg/ml)and confirmed by PCR.
- 将ScFvA1-Etag基因，GLS融合基因重组进大肠杆菌-酵母穿梭质粒pPIC9K, 分别构建含有ScFvA1-Etag基因，GLS融合基因的表达载体pPIC9K-ScFvA1-Etag，pPIC9K-GLS。The ScFvA1-Etag and the glucose-linker-ScFvA1 (GLS) fusion gene were cloned into the vector pPIC9K and expressed in methylotrophic yeast Pichia pastoris GS115, respectively, under the control of the AOX1 promoter.
- HBV全基因核心蛋白变异株稳定表达载体的构建及其抗原表达CONSTRUCTION OF STABLE EXPRESSION VECTORS WITH HBV GENOME CAPSID PROTEIN MUTANTS AND THEIR ANTIGENIC EXPRESSION
- 芳香烃受体及其核转运蛋白融合表达载体的构建与序列鉴定Construction and sequence analyzing of aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator
- 共表达载体coexpression vector
- DNA表达载体DNA expression vecotor
- pET表达载体pET expression vector
- 表达载体法siRNA expression vectors
- 原核表达载体prokaryotic expression vector
- 表达载体构建Expression vector construction
- 基因表达载体gene expression
- RNAi表达载体RNAi expression vector
- 双元表达载体binary expression vector
- 酵母表达载体yeast expression vector
