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- 转座载体pUT／mini-Tn5transposon vector pUT/mini-Tn5
- 方法 :将 HGV NS3基因片段定向克隆至转座载体 p Fast Bac HTa,转化 DH10 Bac感受态细胞 ,37℃振荡培养 4h使发生转座 ,用抗生素平皿筛选重组 bacmid。Methods: Recombinant plasmid pFHTNS3 was constructed by inserting HGV NS3 gene into transposing vector pFastBac HTa. pFHTNS3 plasmid was further transformed into DH10Bac competent cells.
- 转to convey
- 座to sit
- 载体carrier
- 白羊座Aries
- 转帐transfer money from|into an account
- 摩羯座Capricornus
- Tn5转座子Tn5 transposon
- 处女座virgo
- 转寄forward
- 轴承座shaft block
- 转矩torque
- 转晴fair
- 座席pew
- 转置transposition
- 座套seating
- 转出roll out
- Mini-Tn5转座子Mini- Tn5 transposon
- 鞍座bicycle saddle
