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- 重组大肠杆菌BL21(DE3)/rbLF-N高密度培养条件优化High cell-density culture of recombinant E.coli BL21(DE3)/rbLF-N
- Mg~(2+)和氨基酸对重组大肠杆菌BL21(DE3)生长及羧肽酶原B表达的影响Influence of Magnesium Ion and Amino Acid on Growth of Recombinant E.coli BL21(DE3) and Expression of Procarboxypeptidase B
- 重组大肠杆菌BL21recombinant
- 重组reform
- 结论IL-24的重组载体在大肠杆菌BL21(DE3)可以表达GST-IL-24融合蛋白。The prokaryotic expression plasmid pGEX-IL-24 was constructed,GST-IL-24 molecular mass was approximately 50 000.Conclusion GST-IL-24 fusion protein was expressed in E. coli BL21(DE3) which was transformed from the recombinant vector of IL-24.
- P185~(c-erBb-2)胞外区结构域在大肠杆菌BL21中的表达分析The Expression Analysis of Extracellular Domain of P185~( c-erBb-2) in E.coli (BL21)
- 大孔离子交换树脂在重组大肠杆菌生产hEGF中的原位分离作用IN-SITU SEPARATION BY MACROSPORE-ANIONIC EXCHANGE RESIN DURING RECOMBINANT E. Coli CULTIVATION FOR hEGF PRODUCTION
- 将PET30bVP2和rBacVP2分别在大肠杆菌BL21株和sf9昆虫细胞中进行表达。coli BL21 strain and sf9 insect cell.
- 资产重组reorganized assets; assets reorganization
- 重组大肠杆菌分批-补料培养生产类人胶原蛋白的过程控制A study on the process control for production of human-like collagen in fed-batch culture of escherichia coli BL
- 将gfpxm克隆至pTO-T7表达载体,GFPxm在大肠杆菌BL21中的表达量达菌体总蛋白的50%左右。The entire coding sequence was cloned into the pTO-T7 expression vector and expressed in E coli BL21. The expression yield of GFPxm was amounted to 50%25 of the total protein. Compared with GFP of A.
- 企业重组reshuffle of the enterprises
- 利用表达载体pET-22b(+),实现了色褐链霉菌磷脂酶D基因在大肠杆菌BL21(DE3)中的高效表达。In this research,the gene encoding phospholipase D from Streptomyces chromofuscus was cloned into expressed vector pET-22b(+) constituting recombinant plasmid pET-pld which was tranformed into E. coli BL21(DE3).
- 重组大肠杆菌高密度发酵生产类人胶原蛋白的过程控制研究Process Control for Production of Human-like Collagen in Fed-batch Culture of Escherichia coli BL
- 具有高谷胱甘肽合成活性重组大肠杆菌的构建及合成反应过程CONSTRUCTION OF RECOMBINANT E. COLI WITH HIGH GLUTATHIONE BIOSYNTHETIC ACTIVITY AND THE BIOSYNTHETIC PROCESS
- 岸蟹金属硫蛋白在大肠杆菌BL21中的表达Expression of Carcinus maenas Metallothionein Gene in Escherichia Coli BL21
- 应用溶氧反馈控制高密度培养重组大肠杆菌过程中乙酸的产生Preventing Acetate Production in High Cell Density Cultivation of Recombinant Escherichia coli by Using Feedback Control of Dissolved Oxygen
- 大肠杆菌BL21(DE3)E. coli BL21 (DE3)
- 重组大肠杆菌分批-补料高密度发酵生产类人胶原蛋白的动力学Kinetics of High Cell Density Fed-batch Culture of Recombinant Escherichia coli Producing Human-like Collagen
- 重组大肠杆菌recombinant Escherichia coli