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- 重组质粒pGEX-Csn转化E.Recombinant plasmid pGEX-Csn was transformed into E.
- 结果构建了重组质粒pGEX-4T-CTB,CTB基因片段分子量约为376bp;Finally,the recombinant plasmid pGEX-4T-CTB was successfully constructed with a CTB gene fragment of 376 bps.
- 将重组质粒pGEX一SAGI转化大肠杆菌BL21一CodonPlus(DE3)一RP菌株中，在IPTG诱导下表达;The recombinant plasmid of pGEX-SAGl was transformed to a bacterium BL21-Codon Plus(DE3)-RP and the recombinant protein was expressed under the inducement of IPTG.
- 用IPTG诱导重组质粒pGEX-2T/htL-PGDS表达的最佳浓度为1 mmol/L,最佳时间为4 h.The optimal concentration of IPTG, which can induce the recombina nt pGEX -2T/ht L-PGDS to express fusion GST/htL-PGDS protein , is 1mmol/L, and the o ptimal induction period is 4h.
- 将报告基因绿色荧光蛋白基因(gfpmut3a)分别置于recA和uvrA启动子调控下,构建成质粒pRecAgfp和pUvrAgfp,并转化E.Plasmids pRecAgfp and pUvrAgfp were constructed after DNA damage-inducible promoters of recA and uvrA from Escherichia coli were fused to the reporter gene gfpmut3a operon.
- 方法用PCR方法扩增ctxB目的基因片段,克隆至pQE30-napA质粒的napA基因上游,构建含双基因的表达质粒pQE30-napA-ctxB(pQE30-nctB),经测序分析确认后转化E.Methods The ctxB gene was amplified by PCR and cloned into plasmid pQE30-napA. The recombinant plasmid was identified by sequencing, and then transformed into E.
- 重组质粒dna recombinant plasmid
- 旋毛虫ES抗原特异性蛋白两个结构基因的序列分析及重组质粒的构建SEQUENCING OF TWO STRUCTURAL GENES ENCODING SPECIFIC PROTEINS IN ES ANTIGEN FROM TRICHINELLA SPIRALIS AND CONSTRUCTION OF RECOMBINANT PLASMIDS
- 方法设计引物,采用PCR法分别扩增UreB表位多肽编码基因Uepi和LTB编码基因,重叠延伸PCR法将两段基因拼接,T-A克隆后,构建融合基因表达质粒pET-22b(+)-Uepi-LTB,经酶切鉴定后转化E.Methods Amplify the genes encoding UreB epitope polypeptide and LTB by PCR respectively and link by overlap extension PCR. After T-A cloning,the linked gene Uepi-LTB was cloned into prokaryotic expression vector pET-22b(+),and the constructed recombinant plasmid pET-22b(+)-Uepi-LTB was transformed to E.
- 本研究对含有BDV p2 4重组质粒PGEX 3X的大肠杆菌表达系统进行了优化表达BDV p2 4蛋白 ,在IPTG 2mmol/L、3h表达量最大 ,同时用BDV p2 4单克隆抗体证实了其特异性。A method for BDV p24 specific antibody detection was established based on experiments by carrying out optimization of expression of BDV p24 protein expressed by recombinant plasmid PGEX 3X containing BDV p24 in E. coli . The expression was the highest at 2mmol/L of IPTG in 3h.
- 前列腺特异性膜抗原启动子调控的重组质粒的构建及表达The Construction of Recombinant Plasmid with Prostate-specific Membrane Antigen Promoter Controlling Reporter Gene Expression
- coliBL21中，利用转化子在RBB一木聚糖平板上形成的透明圈，分别筛选到含有重组表达质粒pET一BCxE和重组表达质粒pGEX一BcXE的阳性表达子E.coli BL21. Two positive recombinants, E. colt BCE5 containing pET-BCXE vector and E. coliBCE1 harboring pGEX-BCXE vector, were obtained, which forming zones of clearing on RBB-xylan plates.
- 重组质粒构建construction of recombinant plasmid
- 水稻叶绿体DNA克隆片段的分析和rbcL基因在重组质粒上的定位Characterization of A Cloned DNA Fragment of Rice Chloroplast and Location rbcL Gene in the Recombinant Plasmid
- TIMP-1重组质粒TIMP-1 recombinant plasmid
- 氧化硫硫杆菌重组质粒pSDT125的构建及其在大肠杆菌之间的转移CONSTRUCTION OF THIOBACILLUS THIOOXIDANS RECOMBINANT PLASMID pSDT125 AND ITS MOBILIZATION AMONG E. COLI STRAINS
- 为了实现蛋白内含肽(Intein)介导的重组环状胸腺五肽结构类似物[cyclo-(Cys-Arg-Lys-Asp-Val-Tyr-),cTP]的高效制备,设计并合成编码6个氨基酸的cTP基因,克隆到表达载体pTWIN1,重组表达质粒pTW-cTp转化E.In order to obtain the recombinant Tp-5 cyclic analogue [cyclo-(Cys-Arg-Lys-Asp-Val-Tyr-),cTP]efficiently by intein-mediated single column purification, a gene encoding 6-amino acids peptide was designed, synthesized and cloned into Escherichia coli expression vector pTWIN1. The recombinant vector pTW-cTP was transferred into E.
- 重组质粒pRSETDNAThe recombinant plasmid pRSET DNA
- pSH-CUP重组质粒构建constructing the recombinant plasmid pSH-CUP
- 微囊化人类心房肽cDNA重组质粒转染细胞对实验性高血压大鼠肾组织学改变的影响Effects of Encapsulated Plasmid Recombining with hANP cDNA Transfected Cells on Morphological and Histological Characteristic of Experimental Hypertensive Rats
