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- 重组NP蛋白recombinant nucleoprotein(NP)
- 自H5N1阳性病料中提取总RNA,反转录后采用高可信度DNA聚合酶,经PCR扩增NP基因,采用Invitrogen定向表达系统进行克隆表达,纯化获得N末端携带多聚组氨酸标签的重组NP蛋白,分子质量约66.0ku。Total RNA was extracted from positive clinical sample of H5N1 subtype of avian influenza virus, NP gene was amplified by reverse transcriptase-polymerase chain reaction using high proofreading polymerase (PyobestTM DNA Polymerase), and expressed using Invitrogen championTM pET directional TOPO expression system. Recombinant protein containing polyhistidine (6xHis) tag in N-terminal was about 66.0 ku in size, had been obtained and purified.
- 重组reform
- 资产重组reorganized assets; assets reorganization
- NP蛋白NP protein
- 企业重组reshuffle of the enterprises
- 禽流感病毒NP蛋白avian influenza virus nucleoprotein
- 重组N蛋白recombinant N protein
- 重组E蛋白Equine West nile virus antibody
- 重组M蛋白recombinant M protein
- 重组A蛋白recombinant protein A
- 重组S蛋白S proteins
- 重组Cap蛋白recombinant Cap protein
- E2重组蛋白E2 recombinant protein
- 重组GP5蛋白recombinant GP5 protein
- 重组P1蛋白recombinant P1 protein
- 重组P25蛋白recombinant P25 protein
- 重组VP2蛋白recombinant VP2 protein
- 重组VP7蛋白recombinant VP7 protein
- 重组gD蛋白recombinant gD protein
