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- (二)实验方法:每位入选患者抽取外周静脉血1.0ml,以TaKaRa全血DNA提取试剂盒提取DNA,以exon3、exon4及exon7的目标片断为模板进行PCR扩增,然后对扩增产物进行基因测序。Methods: Took 1.0ml venous blood from every recruited patient, extrac-ted DNA with TaKaRa whole blood Genome kit, PCR amplified the objective fragments of exon3, exon4 and exon7, genotyped all the amplified fragments.
- DNA提取试剂盒DNA extraction kit
- 胰腺组织RNA含量丰富 ,极易降解RNA ,操作速度、RNA提取试剂盒的质量和防止RNA污染的综合措施是成功制备高质量RNA的关键The pancreas in rat is abound in Rnases, which makes RNA in pancreas stay in high risk of degradation. The important factors for gaining high quanlity of RNA from pancreas are processing quickness of preparation RNA and the quality of reagent.
- 动物组织总蛋白提取试剂T-PER Tissue Protein Extraction Reagent
- 落叶松-杨栅锈菌DNA提取新方法研究A renewed DNA extraction method for molecular study of Melampsora larici-populina
- 从农用钾肥中提取试剂级氯化钾的工艺条件研究Extraction of Analytical Reagent Potassium Chloride from Agricultural Potassium Manure
- 福尔马林固定石蜡包埋组织DNA提取方法对pcr的影响Extraction of DNA from formalin fixed and paraffin embedded tissues and its effect on PCR
- 全国人体重要寄生虫病现状调查中囊虫病试剂盒批间差异的比较Comparison of difference among three groups of cysticercosis kit in the national survey on the important parasitic diseases
- DNA提取DNA extraction
- 诊断试剂盒diagnostic reagent kit
- 总DNA提取DNA extraction
- 两种方法均以硼砂为缓冲液,分别用SDS、和CTAB为主要提取试剂,可以提取出高质量的木本植物总RNA;The main reagent of extraction buffer is borax, and the main extraction reagent is SDS and CTAB respectively in the two extraction methods.
- 食品安全和反兴奋剂领域常用药物快速检测试剂盒的开发Development of ELISA Kats and gold test strips in food safety and performance-enhancing drugs
- DNA提取量Amount of extracted DNA
- T3试剂盒Trilute
- 胚乳DNA提取endosperm DNA extraction
- 钙试剂盒Calcium
- DNA提取方法DNA extraction
- PCR试剂盒PCR kit
- 微量DNA提取mini-exaction DNA