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- RAPD扩增RAPD
- 以改进的CTAB法获得了较高质量的胡枝子属植物基因组DNA，可以很好地用于RAPD扩增研究。With the improved CTAB protocol, high quality genome DNA of the Lespedeza populations were obtained for RAPD amplification.
- 说明改良的CTAB法能成功用于梨属植物DNA提取,建立的最佳反应体系可用于梨属植物RAPD扩增。It explained that reformed CTAB method could extract the genomic DNA of Pyrus successfully,and the optimum RAPD reaction system could amplificate RAPD in Pyrus L..
- 结果表明,在RAPD扩增中存在着大量的错配,这些错配的产生有很大的随机性,这使扩增中错配结合的方式很多;Elena took a series experiments of RAPD to study the kind of the mismatch.
- 应用44种随机引物对10种麦红吸浆虫滞育虫态进行RAPD扩增,共筛选出6条随机引物S1,S50,S81,S83,S1031,S1248可以对10种麦红吸浆虫滞育虫态扩增出稳定清晰的多态性片断(附图1)。The genomic DNA of ten samples was amplified with 44 random primers. Only six primers (S1, S50, S81, S83, S1031, S1248) could produce clear fragments and obtained a total number of 81 fragments in ten samples amplified (Fig.1).
- 扩增性Proliferation
- 结果表明除SDS法外,其余两种方法均可获得完整的海带基因组DNA,RAPD扩增谱带清晰稳定。 尤以改良CTAB法提取的DNA质量较好。The result was that the latter two methods could be used for extracting genomic DNA from sporophyte of Laminaria, and that the improved CTAB method was the best ones through which the production had the highest purity.
- 扩增图谱amplification map
- cDNA扩增cDNA amplification
- 扩增条带Amplified band
- 种质扩增Enhancement
- 分段扩增PCR
- 扩增突变trinucleotide repeat expansion
- 酶（促）扩增enzymatic amplification
- 扩增和缺失expansion and contraction instability
- 单管PCR扩增one tube PCR amplification
- 干细胞扩增stem cell expansion
- PCR扩增条件PCR amplification conditions
- 选择性扩增Selective amplification
- 多重耐药株以及敏感株acrAB基因扩增产物无点突变和缺失。No deletion or point mutation in acrAB gene were found in Mar and susceptible clinical Escherichia coli isolates.
