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- 人类端粒酶反转录酶human telmerase reverase transcriptase
- 合成的方案是采用先合成AGD、CGG和DAG三个三核苷二磷酸片段,然后再以AGD+pCGGDAG(3+p6)或AGDCGG+pDAG(6+p3)两种方式用T_4 RNA连接酶连接成为所需要的九核苷酸。As the first step, three trinucleoside diphosphate fragments AGD, CGG and DAG were synthesized and then, in the presence of T_4 RNA ligase, joined to form the desired nonamer either through route 3+ p6 (AGD+ pCGGDAG) or route 6+p3 (AGDCGG+pDAG). The phosphodiester methed was used in the syntheses of CGG and DAG;
- 用反转录PCR(RT-PCR)检测转染细胞ATF基因的表达;Then the expression of ATF was analyzed by means of reverse transcription polymerase chain reaction (RT-PCR).
- RNA连接酶反转录PCRRNA ligase reverse transcrip-tion-PCR
- 反anti-
- 评价反转录多聚酶链反应检测大肠癌患者血清人端粒酶反转录酶mRNA表达的敏感性及特异性:对照组比较Sensitivity and specificity of human telomerase reverse transcriptase mRNA expression in the serum of patients with colorectal carcinoma: a controlled comparison using reverse transcription polymerase chain reaction
- 泛素连接酶ubiquitin ligase, ubiquitinligating enzyme
- 巢式反转录PCRNested RT-PCR
- 辅酶A连接酶CoA ligase
- 端粒酶反义核酸telomerase antisensenucleic acids
- 泛素连接酶E3Ubiquitin ligase E3
- 荧光定量反转录PCRFluorescence quantitative reverse transcription PCR (FQ RT-PCR)
- 碳氮连接酶类Carbon-nitrogen ligases
- 多聚半乳糖醛酸酶反义基因anti-polygalacturonase gene
- α半定量反转录PCRSemi-quantitative RT-PCR
- E3泛素连接酶E3 ligase
- 端粒酶反义寡聚脱氧核苷酸telomerase antisense oligodeoxy nucleotides
- 连接酶合成产物的瞬时速度与细胞中可利用的ATP含量成比例。The degree of bond formation by ligases is proportional to the amount of ATP available in the cell at a particular instant.
- 猪内源性反转录病毒5'端非编码区的克隆及结构分析Cloning of Porcine Endogenous Retrovirus 5'-Untranslated Region and Analysis of its Structure
- 反转录PCRreverse transcriptional PCR; RT-PCR
