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- 用Cfol和BamHI将含有E2基因的3.2kb片段从HPV16基因组中切出来,用S1核酸酶修平末端;coli. The 3.2kb fragment containing the E2 gene was cut out from HPVl6 genome and blunted with nuclease S1. The plasmid pBD2 DNA was linearized with Hindi and blunted with nuclease S1 too.
- 采用Trizol试剂对病毒进行了核酸提取,电泳结果发现有4条核酸条带,分别为3.0kb、1.3kb、0.8kb、0.75kb,对RNA核酸酶、S1核酸酶敏感,对DNA酶抵抗,为单链RNA病毒。The nucleic acid of virus was extracted by using the Trizol reagents and analyzed with 1%25 agarose electrophorosis. It shows the nucleic acid has four bands sized 3.0, 1.3, 0.8 and 0.75 kb respectively, and is sensitive to RNase and S1 nuclerase and resistant to DNase, indicating these bands as single?strand RNA.
- 核酸酶水解核酸的酶，如核酸内切酶和核酸外切酶Any of several enzymes, such as endonucleases and exonucleases, that hydrolize nucleic acids.
- 酶的enzymatic
- 识别序列为非回纹对称结构限制核酸酶的正确切割位点The True Recognizing Sequence of the Restriction Enzyme Whose Recognizing Sequence is Nonpalindromic
- 辅酶coenzyme
- 酶解zymohydrolysis
- 过氧化氢酶catalase
- 金黄色葡萄球菌核酸酶在大肠杆菌中的表达及其活性分析Expression and nuclease activity analysis of staphylococcus nuclease in E. Coli
- 酶活性enzyme activity
- 禽腺病毒贵州分离株HS-1的核酸酶切分析与蛋白抗原鉴定Antigenicity identification and DNA restriction map analysis of Guizhou isolate HS-1 strain
- 核酸酶P1nuclease P1
- S-核酸酶S-RNase
- Ｓ１核酸酶作图S1 nuclease mapping
- 限制性核酸内切酶restriction endonuclease
- 核酸酶nuclease
- 脾外切核酸酶spleen phosphodiesterase
- BAL 31核酸酶BAL 31 nuclease
- S1核酸酶S1 nuclease
- Ter核酸酶Ter nuclease
