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- T7RNA聚合酶表达系统T7 RNA polymerase expression system
- T7RNA聚合酶T7 RNA polymerase
- 自H5N1阳性病料中提取总RNA,反转录后采用高可信度DNA聚合酶,经PCR扩增NP基因,采用Invitrogen定向表达系统进行克隆表达,纯化获得N末端携带多聚组氨酸标签的重组NP蛋白,分子质量约66.0ku。Total RNA was extracted from positive clinical sample of H5N1 subtype of avian influenza virus, NP gene was amplified by reverse transcriptase-polymerase chain reaction using high proofreading polymerase (PyobestTM DNA Polymerase), and expressed using Invitrogen championTM pET directional TOPO expression system. Recombinant protein containing polyhistidine (6xHis) tag in N-terminal was about 66.0 ku in size, had been obtained and purified.
- DNA聚合酶DNA polymerase
- T4DNA聚合酶T4DNA polymerase
- 淋巴系统lymphatic system
- 磷酸酶phosphatase
- II型系统type-II system
- 0L系统zero-sided Lindenmayer system
- T系统transverse tubular system
- (使)聚合polymerize
- Q酶branching enzyme
- 2D系统2D system
- 高聚合凝聚剂high polymer coagulant
- MK系统MK system
- [生化]合酶synthase
- 磷酸聚合phosphoric acid polymerization
- DNA酶deoxyribonuclease
- RC系统Ritchey-Chretien system
- RNA酶ribonuclease