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- Taq酶量Taq enzyme
- Taq聚合酶Taq polymerase
- TaqⅠ/PstⅠTaqⅠ/PstⅠ
- 用Taq Man探针实时定量PCR法快速检测鼠疫耶尔森菌Rapid detection of Yersinia pestis with TaqMan probe
- 转化宿主菌BL21(DE3),经IPTG诱导、SDS-PAGE分析表明目的蛋白得到表达; 且能与His-Taq单抗相结合。coli BL21(DE3) and induction by IPTG and SDS-PAGE indicated that the target protein was expressed.
- 方法:选用不同的引物、Mg~(2+)、模板浓度、Taq聚合酶用量等,获得一优化的反应体系以对白假丝酵母菌进行RAPD分型。Methods Candida albicans was geno- typed using RAPD under the different conditions of primer,template,concentration of Mg~(2+)and dosage of Taq polymerase.
- 方法 利用实时定量PCR(Taq Man)方法检测 8例CML患者外周血单个核细胞、3例CML患者CD4 + 和CD8+ 细胞中TRECs的水平。Methods Quantitative detection of T cell receptor excision DNA circles (TRECs) in DNA of peripheral blood mononuclear cells from 8 cases with CML, CD4 + or CD8 + cells from 3 cases with CML were preformed by real time PCR (TaqMan) analysis.
- 方法在提取纯化美花石斛基因组DNA的基础上,利用PCR扩增技术和方法,对Mg2+,dNTP,模板DNA,引物,Taq聚合酶等反应条件进行优化。MethodsSome reaction condition was optimized by amplifying technology and method of PCR based on isolating genomic DNA from D.
- 以百山祖冷杉DNA为材料,分析了DNA浓度、Mg2+浓度、dNTP浓度、Taq酶的含量以及退火温度对ISSRPCR扩增结果的影响,经过优化实验建立了百山祖冷杉ISSRPCR最佳反应体系.Factors which affect the ISSR analysis of Abies beshanzuensis,such as annealing temperature and concentrations of template DNA,Taq DNA polymerase,Mg~2+ and dNTP,etc. were examined.
- 以高粱细胞质雄性不育系和保持系 A1Tx6 2 3 A/B,A2 V4 A/B为材料 ,考察了 Mg2 + ,Taq酶 ,d NTP,引物 ,模板等的用量、循环参数及不同的 PCR仪对随机扩增多态性 DNA(RAPD)反应的影响。CMS sorghum A 1T x623A/B and A 1 V 4A/B as plant material were used in present experiment for optimizing the factors influencing RAPD reaction effect.
