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- TopoⅡTopo Ⅱ
- Topo-ⅡTopo- Ⅱ
- TOPO-ⅡαTOP-Ⅱα
- TopoⅡαTopo Ⅱα
- Topo-ⅡaTopo-Ⅱa
- TOPO克隆TOPO cloning
- 新荧光体系Sm-DBM-TOPO-SDS的研究和应用SPECTROFLUORIMTRIC STUDIES ON THE NEW FLUORESCENT SYSTEM OF SAM ARIUM-DBM-TOPO-SDS
- 探讨了TOPO(TX-100)-SDS对Ln-槲皮素二元体系的增敏机理。The enhancement mechanism of Ln quercetin binary system by TOPO(TX 100) SDS was discussed.
- 本文研究了三辛基氧化膦(TOPO)学修饰电极预富集石墨炉原子吸收法测定水样中痕量锑。A preconcentration on TOPO chemically modified electrode-graphite furnace AAS method has been developed for the determination of trace antimony in water samples.
- 为了探讨F基因在DNA免疫防制新城疫(ND)中的免疫原性,将NDV Z株F基因插入到真核表达载体pcDNA3.1/V5-H is-TOPO中,构建了真核表达质粒pcDNA NDV ZF。To assess the probability of F gene DNA vaccine in controlling ND,the NDV F gene was inserted into eukaryotic expression vector pcDNA3.1/V5-His-TOPO,and an eukaryotic expression recombinant plasmid pcDNA NDV ZF,which containing start codon and stop codon,was constructed.
- 用RT-PCR从经ConA刺激的马外周血单个核细胞(PBMC)中扩增出马白细胞介素18(Equine interleu-kin-18,EIL-18)前体蛋白(precursor EIL-18,pEIL-18)基因的cDNA,然后克隆至载体pCR2.1-TOPO中,鉴定并命名为pCR2.1-pEIL-18。Using the total RNA extracted from ConA stimulated equine peripheral blood mononuclear cells(PBMC) as template,the cDNA of interleukin l8 was amplified by RT-PCR. The cDNA was subsequently cloned into the vector pCR2.1-TOPO and sequenced.