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- U6启动子U6 promoter
- 方法构建利用U6启动子转录功能性小干扰RNA(siRNA)的质粒载体,对阳性克隆进行酶切和测序鉴定后,转染人甲状腺癌FRO细胞,利用RT-PCR和W eston blot法检测hTERT基因的表达情况。Methods A vector containing U6 promoter used to transcribe functional small interfering RNA(siRNA) targeting hTRET gene was constructed. After identification by restriction enzyme digestion and sequencing,the plasmid of positive clones was transfected into human thyroid carcinoma FRO cell line. The expression of hTRET gene was detected by RT-PCR and Western blot.
- 方法:设计和构建由U6启动子驱动产生hTERT的siRNA表达质粒,转染至ALVA-41前列腺肿瘤细胞,以TRAP-ELISA方法观察细胞端粒酶活性,W estern-b lot检测端粒酶蛋白的表达,细胞记数法检测对细胞生长的影响。Methods:A plasmid including U6 promoter and siRNA of hTERT was designed and constructed. The plasmid was transfected into ALVA-41 cell line. The telomerase activity was tested by telomerase repeat amplification protocol ELISA(TRAP-ELISA).
- 子midnight
- 启动to start
- 重新启动reboot
- 人U6启动子human U6 promoter
- 启动子promotor
- 可及启动子accessible promoter
- T7启动子T7 RNA polymerase promoter
- TK启动子TK promoter
- 基因外启动子extragenic promoter
- 小启动子minimal promoter
- A3启动子actin 3 promoter
- C区启动子core promoter
- KDR启动子KDR promoter
- 35S启动子35S promoter
- E8启动子E8 promoter
- AFP启动子AFP promoter
- GAP启动子GAP promoter
