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Methods The tumor antigen peptides from 4T1 breast cancer cells were acquired by freeze thawing method and HSP70-4T1 peptides complex was reconstructed in vitro. 方法冻融法获取BALB/C小鼠4T1乳腺癌细胞抗原肽,体外重组HSP70-4T1抗原肽复合物。
Recent researches have demonstrated that the ATPase domain of HSP70 and the tumor antigenic peptide that binds to Hsp70 were the crucial parts eliciting tumor-specific immunity. 最近的研究表明HSP70携带的小肽及其ATPase结构域是HSP70抽提物激活肿瘤特异性免疫反应的关键。
Methods Tumor antigen peptides from elemene-combo Hca-F cell (HTA) combined with HSP70BCG into HTA-HSP70BCG in vitro. DCs were induced in medium with GM-CSF and IL-4 and pulsed with HTA-HSP70BCG, HTA and HSP70BCG, respectively. 方法来源于小鼠的肝癌Hca-F榄香烯复合疫苗的抗原(HTA)与卡介苗来源的HSP70(HSP70BCG)在体外构建成HTA-HSP70BCG复合物,用GM-CSF和IL-4诱导树突状细胞(DCs),分别用HTA-HSP70BCG、HTA和HSP70BCG对其冲激。
Xu: It will become a tumor antigen. 徐：它会变成一种肿瘤抗原，
In vivo and in vitro activation of CTL by murine dendritic cells pulsed with acid eluted tumor antigen peptides 弱酸洗脱提取的肿瘤抗原肽致敏的树突状细胞对CTL的体内外激活效应
Cytotoxicity Induced by Dendritic Cells Pulsed with Acid Eluted Tumor Antigen Peptides against Bladder Cancer Cell Line 负载酸洗抗原肽的树突状细胞诱导抗膀胱癌的作用
Methods:Human TSN,inactivated TSN and tumor antigen were prepared. 方法 :制备人肺腺癌A5 4 9细胞的TSN、灭活TSN和冻融抗原 ;
Tumor antigenic peptide 肿瘤抗原肽
Tumor antigen specific CTLs may prevent metastasis in the LCI-D20 model and prolong the survival time. 肿瘤抗原特异性CTL可以预防LCI-D20模型肝癌发生转移,延长动物存活时间。
Objective To study the immune response of dendritic cells (DC) modified with tumor antigen and SEB to transplanted tumor in nude mice. 摘要目的研究结肠癌患者外周血树突状细胞(DC)体外诱导后在裸鼠体内的抗癌效应。
Somatostatin receptor-like immunoreactivity was in most cases located at the plasma membrane as well as in the cytoplasm of the tumor cells and was completely blocked with antigenic peptide. 良性卵巢上皮肿瘤组织中大部分细胞的胞浆和胞膜都呈强染色，呈棕褐色。
Objective To investigate antitumor effects induced by dendritic cells (DCs) pulsed with complex of tumor antigen from elemene-combo tumor cell vaccine-heat shock protein 70 of BCG (HTA-HSP70BCG). 目的观察体外构建的榄香烯复合瘤苗抗原-卡介苗热休克蛋白70复合物(HTA-HSP70BCG)诱导的树突状细胞疫苗的抗肿瘤效应。
Aβ1-15 multiple antigen peptide vaccine Aβ1-15多重抗原肽疫苗
It will become a tumor antigen. 它会变成一种肿瘤抗原。
human soluble antigen peptide 35(HS-Ag P35) 人视网膜可溶性抗原第35段抗原决定簇
METHODS: BMDCs were stimulated by CpG ODN in combination with tumor antigen (TAg). The expression of CD80 on BMDC surface was analyzed by FCAS. Level of IL-12 (p70) in supernatants of BMDC culture was detected by ELISA. 方法 :CpGODN联合肿瘤抗原 (TAg)体外刺激BMDC ,流式细胞术检测受刺激BMDC表达CD80的阳性率 ,ELISA检测受刺激BMDC分泌IL - 12 (p70 )的水平 ,MTT法检测活化BMDC刺激T细胞的增殖能力 ,联合CpGODN和TAg体内预激小鼠获得CTL ,检测CTL对肝癌细胞株Hca-F的特异性杀伤作用。
The tumor specific CTL was generated form activated autologous T cell by DC vaccine loaded with tumor antigen, and the killing activity of CTL to CT26 cells was tested by the lactate dehydrogenase (LDH) release assay in vitro. 负载肿瘤抗原的DC疫苗激活同源T淋巴细胞产生细胞毒性T淋巴细胞(CTL),采用乳酸脱氢酶(LDH)4h释放法检测CTL在体外对CT26细胞的杀伤活性;
The binding of antigenic peptide sequences to major histocompatibility complex（MHC） molecules is a prerequisite for stimulation of cytotoxic T cell responses. 抗原多肽序列与主要组织相容性复合体(MHC)分子的结合是引起T细胞免疫应答的先决条件。
It is critical in antitumor immune responses that the APCs (antigen presenting cells) present tumor antigens(Tags) to T cells to activate immune system to eradicate tumor cells. 抗肿瘤免疫应答中关键的一步是抗原递呈细胞(APC)将肿瘤抗原递呈给T细胞，激活机体的免疫系统来清除肿瘤细胞。树突状细胞(DC)是目前认为功能最强的抗原递呈细胞，也是APC的一个独特的亚群。
The adoptive transfer technique uses a small fragment of each patient's melanoma to grow T-cells in vitro targeting specific tumor antigens. 采用转移技术利用每个患者黑色素瘤中的极少部分，体外培养特异肿瘤抗原T细胞。