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- The optimized strategy for the amplifying and screening of human-mouse phage Fab antibody library. 第二部分:噬菌体展示F ab抗体库构建和筛选条件的优化。
- Goat anti human IgG Fab antibody affinity chromatography column was prepared and the Fab supernant was purified. 制备羊抗人IgGFab抗体亲和层析柱、纯化Fab片段。
- The recombinant Fab antibody has been purified, and the molecular, glycosylation and affinity activity have been analized. 1mg重组Fab抗体相当于40U的抗乙肝表面抗原抗体(20U/mg).
- By guided-selection and chain shuffling technique, the humanized Fab antibody against HAb18G is obtained, which lays the foundation for further research. 利用导向选择链更替技术 ,筛选出特异性较强、完全人源化的抗肝癌抗原HAb18G的Fab抗体 ,为进一步的工作打下了基础。
- Anti Fas Fab phage antibody displaying on phage surface and soluble anti Fas Fab showed specific binding activity to Fas by ELISA. Conclusion The successful preparation of anti Fas Fab antibody paved a way for further study and application. 结论制备的可溶性抗Fas?Fab抗体具有抗Fas抗体的特异性,为进一步的研究奠定了基础。
- This article is using phage display technology constuncted a naive library of phage-display human Fab library. Obtained the completely humanized Fab antibody against idiotypic antibody of schistosoma japonicum from large naive phage-display library. 本文应用噬菌体抗体库技术，构建大容量天然人源Fab抗体库，从中富集、筛选全人源日本血吸虫抗独特型抗体并进行初步鉴定，为研制可用于人体血吸虫病免疫预防的抗独特型抗体疫苗奠定基础。
- AIM: To construct a universal expression vector for human-mouse chimeric Fab antibody and to use it for construction and expression of human-mouse chimeric Fab antibody against human hepatoma associated antigen HAb18G. 目的:构建一个在大肠杆菌中表达人鼠嵌合Fab抗体的通用载体;并用于抗人肝癌相关抗原HAb18G人鼠嵌合Fab抗体的表达.
- The set of optimized family-specific primers can amplify the human genes of the Fd and light chains with a high efficiency. These will be helpful for the construction of human Fab antibody libraries. 通过本部分工作，在人抗体可变区胚系基因家族分类基础上，根据其5’端保守序列重新分组，优化设计特异性引物，可以保证扩增效率，有利于大容量人源噬菌体Fab抗体库的构建和筛选。
- Methods Construct and pan human Fab antibody library to influenza virus Sydney strain (H3N2) from vaccinated donors by using phage display technology and secreted expression in \%E. 用纯化的流感病毒A Sydeny 5 97(H3N2 )为固相抗原筛选抗体Fab段 ,并在大肠埃希菌中进行分泌性表达。
- Wu BP, Zhang YL, Xiao B, et al.Construction and screening of natural immune Fab antibody phage library from patients with colorectal cancer[J].J First Mil Med Univ, 2001, 21(4): 270-4. [1]吴保平;张亚历;肖冰;等.;人源性大肠癌自然致敏噬菌体抗体Fab呈现库的构建与筛选[J]
- Enzyme analysis of these Fab antibodies was accomplished for diversity. 筛选获得了结合血管内皮细胞生长因子( VEGF)的人噬菌体 Fab抗体 ;并对所获抗体的多样性进行了进一步分析 .
- ObjectiveTo analyze genes of Fab antibodies against Clonorchis sinensis CAg. 目的 对获得的抗华支睾吸虫循环抗原Fab抗体克隆进行基因分析。
- digoxin-specific fab antibody frag-ments 地高辛特定fab抗体片段
- Digoxin-specific fab antibody fragments 地高辛特定fab抗体片段
- Mouse/human chimeric Fab antibody expression vector was constructed and expressed on the surface of phage successfully. The Fab antibodies had binding activity against HFRS virus antigen. 成功构建了鼠 /人嵌合 Fab噬菌体抗体表达载体 ,并在噬菌体表面表达出可结合 HFRS病毒抗原的鼠 /人嵌合 Fab抗体
- Conclusion The specific binding of Fab antibodies to HCMV was demonstrated by ELISA, IFA and neutralizing activities. 结果 克隆和表达了 3株抗HCMV人源Fab抗体 ,经ELISA证明均具有抗原结合活性 ,间接免疫荧光试验证明具有较高的特异性 ,最后通过病毒中和试验证明其中 2株具有中和活性。
- Study of Developing Human Fab Antibody Against SARS Virus 抗SARS病毒人源Fab抗体的制备研究
- Preparation of Human Antikeratin Fab Antibody Liposomes 人源性抗角蛋白Fab抗体脂质体的制备
- Characterization analysis of recombinant humanized anti-HBsAg Fab antibody 重组人源性抗HBsAg Fab抗体的特性分析
- Expression of human Fab antibody against keratin in E.coli and its renaturation 抗角蛋白Fab抗体在大肠杆菌中的表达与复性研究
