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- Methods Construct and pan human Fab antibody library to influenza virus Sydney strain (H3N2) from vaccinated donors by using phage display technology and secreted expression in \%E. 用纯化的流感病毒A Sydeny 5 97(H3N2 )为固相抗原筛选抗体Fab段 ,并在大肠埃希菌中进行分泌性表达。
- The set of optimized family-specific primers can amplify the human genes of the Fd and light chains with a high efficiency. These will be helpful for the construction of human Fab antibody libraries. 通过本部分工作,在人抗体可变区胚系基因家族分类基础上,根据其5’端保守序列重新分组,优化设计特异性引物,可以保证扩增效率,有利于大容量人源噬菌体Fab抗体库的构建和筛选。
- Optimization of the primers for the construction of human Fab antibody library 人源噬菌体Fab抗体库构建过程中引物的优化和初步鉴定
- Human Fab antibody library 人源Fab抗体文库
- This article is using phage display technology constuncted a naive library of phage-display human Fab library. Obtained the completely humanized Fab antibody against idiotypic antibody of schistosoma japonicum from large naive phage-display library. 本文应用噬菌体抗体库技术,构建大容量天然人源Fab抗体库,从中富集、筛选全人源日本血吸虫抗独特型抗体并进行初步鉴定,为研制可用于人体血吸虫病免疫预防的抗独特型抗体疫苗奠定基础。
- The optimized strategy for the amplifying and screening of human-mouse phage Fab antibody library. 第二部分:噬菌体展示F ab抗体库构建和筛选条件的优化。
- By guided-selection and chain shuffling technique, the humanized Fab antibody against HAb18G is obtained, which lays the foundation for further research. 利用导向选择链更替技术 ,筛选出特异性较强、完全人源化的抗肝癌抗原HAb18G的Fab抗体 ,为进一步的工作打下了基础。
- Screening and identification of the human fab antibody phage display library from the patients with colorectal cancer 人源性大肠癌Fab段噬菌体抗体库的筛选与鉴定
- Selection and Identification of Human Fab Antibody Phage Display Library from Patients with Colorectal Cancer 人源性大肠癌抗体Fab段噬菌体呈现库的筛选与鉴定
- Immunohistochemical stainning about activity of the human Fab antibody phage display library from the patients with colorectal cancer 人源性大肠癌Fab段噬菌体抗体库的免疫组化鉴定
- Screening and identification of a human Fab antibody against idiotypic antibody from a phage-display library of schistosoma japonicum 人源日本血吸虫Fab抗体库的构建及抗独特型抗体的筛选、鉴定
- Screening and identification of human Fab antibody against idiotypic antibody of schistosoma japonicum from large naive phage-display library 大容量天然人源Fab抗体库的构建及日本血吸虫抗独特型抗体的筛选、鉴定
- Study of Developing Human Fab Antibody Against SARS Virus 抗SARS病毒人源Fab抗体的制备研究
- The selection of human monoclonal Fab fragments against HIV-1 gp120 peptide binding chemokine receptor from phage Fab antibody library 人源抗HIV-1gp120趋化蛋白受体结合位点单克隆Fab抗体基因的获得
- Construction and preliminary identification of humanized Fab fragment phage antibody library against Schistosoma japonica 人源性抗日本血吸虫噬菌体抗体库的构建及初步鉴定
- Panning of D-Dimer Specific Fab Antibody From a Phage Antibody Library 从噬菌体抗体库中筛选D二聚体特异的Fab抗体
- Expression of human Fab antibody against keratin in E.coli and its renaturation 抗角蛋白Fab抗体在大肠杆菌中的表达与复性研究
- Generation of human recombinant antibody Fab fragment to influenza virus from combined antibody library. 流感病毒中和性基因工程Fab抗体的研制
- In this project, we constructed a naive human Fab fragment phage display library, provide a platform for human antibody preparation and a basis to new therapy for the malignant tumors. 我们利用噬菌体表面展示技术构建人天然Fab段噬菌体抗体库,建立人源抗体制备技术平台,为寻求恶性肿瘤的免疫治疗新方法打下基础。
- Construction of a Semi-synthetic Antibody Library and Screening Anti-Tie2 Fab Antibody 半合成抗体库的构建及抗Tie2 Fab抗体的筛选