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The molecular weight of the antibiotic substance is 48000 Dalton,tested by sephadex-G75 gel column chromatography. 而且用Sephadex－G75凝胶柱层析法测得抗生物质的分子量为48000道尔顿
Conclusion: Silica gel column chromatography and crystallization are simple and rapid methods to isolate gardenoside. 结论:柱层析和重结晶方法是简单、速分离栀子苷的有效办法。
Methods The compounds were isolated by silica gel column chromatography and identified by spec-tral analysis. 方法通过硅胶柱色谱法分离纯化化合物,谱学方法鉴定其结构。
Then the extracted material was sequently filtered and sperated by means of silica gel column chromatography. 提取物通过硅胶柱层析分离，得到无辣味的红色色素。
Silica gel column chromatography was used to separate and purify total alkaloids from Fritillaria ussuriensis Maxim. 目的:对东北特产平贝母的生物碱进行分离纯化研究。
Conclusion Chrysophanol and physcion could be effectively separated by silica gel column chromatography. 结论硅胶柱色谱可以实现大黄酚和大黄素甲醚的高效分离；
In conclusion,the separation method of silica gel column chromatography applied in this experiment can be used to gain highly purified prod... 结论:采用的硅胶柱层析分离纯化可得到纯度较高的分离组分。
Methods: Silica gel column chromatography and crystallization were used to obtained gardenoside from Gardenia jasminoides. 方法：利用柱层析、重结晶从山栀子的成熟果实中进行分离纯化栀子苷。
The extracts were eluted by 70%,80%,90%ethanol solutions in order in silica gel column chromatography and the refined flovonoids solution was obtained. [方法]采用乙醇回流法提取云南13种蕨类植物根状茎(根)及叶中的总黄酮,经硅胶柱层析,依次用浓度70%25、80%25、90%25的乙醇溶液洗脱,得到精制黄酮溶液,利用紫外光分光光度法测定总黄酮的含量。
Method Compounds were extracted by 90% ethanol-petroleum ether and acetoacetate,then purified by silica gel column chromatography. 方法采用硅胶柱层析进行分离纯化,根据理化性质和波谱学数据进行结构鉴定。
A method is described for successive purification of epigallocatechin gallate(EGCG)and epicatechin gallate(ECG)by medium-pressure silica gel column chromatography. 采用一种连续中压硅胶柱层析分离高纯度表没食子儿茶素没食子酸酯(EGCG)及表儿茶素没食子酸酯(ECG),原料为含量高于98%25的茶多酚。
Pure TS99 could be obtained from pretreatment fermentation broth through macroreticular adsorbent, silica gel column chromatography and thin-layer chromatography. 发酵液经过预处理后，依次用大孔树脂吸附，硅胶柱吸附层析和薄层层析等分离方法即可获得农用抗生素TS99纯品。
Methods Pristimerin was isolated and purified by polyamide column chromatography and silica gel column chromatography,and the content of pristimerin was determined by HPLC. 方法南蛇藤根粉经丙酮冷浸提取后,采用聚酰胺柱层析及硅胶柱层析进行分离纯化;
METHODS Matrine and oxymatrine were isolated from the roots of Sophora tonkinensis by repeated silica gel column chromatography and purified by recrystallization method. 目的从山豆根中精制苦参碱和氧化苦参碱,并以此为化学对照品对山豆根药材进行薄层色谱鉴别。
Methods The petroleum ether extract fraction of Caragana microphylla Lam.was extracted by solvent extraction and isolated by the gel column chromatography. 方法用溶剂法提取,硅胶柱层析法分离得到化合物,通过理化鉴别和光谱法分析化学结构。
MethodsThe petroleum ether extract fraction of Caragana microphylla Lam.was extracted by solvent extraction and isolated by the gel column chromatography. 方法用溶剂法提取，硅胶柱层析法分离得到化合物，通过理化鉴别和光谱法分析化学结构。
In this paper, silica gel column chromatography has been used to separate and purify folic acid from fermented broth. The chromatography method was studied and the suitable conditions were determined. 采用硅胶柱色谱分离纯化生物发酵液中的叶酸,实验研究了色谱方法,确定了适的谱条件。
In the process of experiment, we separated better efficient antioxidative fractions by silica gel column chromatography with different extensible reagent, ethyl acetate: hexane (3:7) and chloroform: methanol (9:1). 实验中采用乙酸乙酯/环己烷(3:7)、氯仿/甲醇(9:1)为展开剂依次二次干柱层析分离出抗氧化物质。
Methods: The compounds wereisolated and purified by macroporous adsorption resin, silica gel column chromatography and preparative TLC chro-matography and their structures were identified on the basis of their physicochemical and spectral data. 方法:利用大孔吸附树脂、硅胶柱层析、制备薄层色谱法进行分离纯化,根据化合物的理化性质和光谱数据鉴定结构。
The methods such as distn. and silica gel column chromatography were used to sep. the various components of the thickener, and the IR spectrometry, GC-MS combination and ESI-MS were used to certify the structures of the different components. 用蒸馏和矽胶柱色谱等方法对增稠剂中的各组分进行分离，并用红外光谱法、气质联用和电喷雾质谱法对各组分进行结构鉴定。