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- 竞争性定量RT-PCRcompetitive quantitative RT-PCR
- 主要包括相对定量RT-PCR(Relative quantitative RT-PCR)、竞争性定量RT-PCR(Competitive quan-titative RT-PCR)、比较定量RT-PCR(Comparative quantitative RT-PCR)和实时定量RT-PCR(Real time quantitative RT-PCR)四种。In particular,it is the most sensitive and reproducible method for the detection of low-abundance mRNA,often obtained from limited tissue samples. In fact,this technique is sensitive enough to enable quantitation of RNA from a single cell. There are four kinds of quantitative RT-PCR: relative quantitative RTPCR,competitive quantitative RT-PCR,comparative quantitative RT-PCR and real time quantitative RT-PCR.
- 目的 建立针对 AML- ETO融合基因转录本的竞争性定量反向 -聚合酶链反应 (RT- PCR)体系 ,并探讨其在 t(8;2 1)急性髓细胞性白血病 (acute myeloid leukemia,AML)微小残留病变跟踪中的应用价值。Objective To construct and evaluate the competitive quantitative RT PCR method for detecting the fusion gene of AML1 ETO in the patients of t(8;21)AML(acute myeloid leukemia).
- 定量RT-PCRQuantitative RT-PCR
- 应用竞争性定量PCR(QC-PCR)技术,对石化废水处理系统中投加的高效芳烃降解菌株Rhodococcus ruber Em1数量变化趋势及其作用进行了分析。A quantitative competitive PCR (QC-PCR) system was developed to quantify the number and analyze the function of the Rhodococcus ruber Em1 strain in a wastewater treatment system in Nanchong oil refinery plant.
- 病毒RNA分子拷贝数和靶基因mRNA水平用实时定量RT-PCR确定。The RNA copies of viral vectors and mRNA levels of target genes were determined by quantitative real-time RT-PCR.
- 比较定量RT-PCRcomparative quantitative RT-PCR
- 应用RT-PCR检测人工感染番鸭体内番鸭呼肠病毒的动态分布Dynamic Distribution of Muscovy duck reovirus in the Challenged Ducks Detected by RT-PCR
- 竞争性PCRcompetitive PCR
- 用定量RT-PCR方法检测脑梗死半影区内凋亡相关基因的表达Quantitative RT-PCR in detecting the apoptosis associated gene expression in penumbra area of cerebral infarction in rats
- RT-PCRRT-PCR
- IC-RT-PCRIC-RT-PCR
- 半定量RT-PCRsemi-quantitative RT-PCR
- 常规RT-PCRconventional RT-PCR
- 实时定量RT-PCR(Real-time PCR)检测Evi-1,MDS1/Evi-1表达。The Evi-1 and MDS1/ Evi-1 genes expression were examined by fluorescent Realtime PCR.
- 竞争性RT-PCRcompetitive RT-PCR
- 定量PCRquantitative PCR ( qPCR)
- PCR定量Quantitative polymerase chain reaction (PCR)
- 半定量PCRSend-quantitative PCR
- 实时定量PCRReal-time PCR
