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- The plasmid was made? by P. Ghosh, who solved the structure of colicin Ia then years ago (published in Nature). A promoter and SD sequence was introduced into the plasmid. 回答:如果克隆序列有翻译开始信号，是可以用来表达的，但是从该论文公布的克隆序列看，没有由方舟子于2006-1-12,02:18:51:
- The plasmid was introduced into rice embryogenic calli by microprojectile bombardment. 用基因枪法将其导入水稻（Oryza sativa L.
- The hut was made of poles covered with grass mats. 茅屋用木杆搭成，上面以草席覆盖。
- The group was made up of doctors. 这个团体是由医生组成的。
- LCB1 gene was cloned into inducible expression vector pYES2 and transformed into Saccharomyces cerevisiae FY2 The plasmid was induced to express with galactose. 酿酒酵母 (Saccharomycescerevisiae)LCB1 (Longchainbase)基因被克隆到酵母诱导表达载体pYES2中 ,并转入到FY2中 ,用半乳糖诱导表达。
- I fancy the car he uses was made in the year one. 我想他用的汽车是很久以前制造的。
- The hut was made out of pieces of wood. 这小屋是用木板建造的。
- She must be made to comply with the rules. 必须强迫她遵守这些规则。
- Methods:A plasmid including U6 promoter and siRNA of hTERT was designed and constructed. The plasmid was transfected into ALVA-41 cell line. The telomerase activity was tested by telomerase repeat amplification protocol ELISA(TRAP-ELISA). 方法:设计和构建由U6启动子驱动产生hTERT的siRNA表达质粒,转染至ALVA-41前列腺肿瘤细胞,以TRAP-ELISA方法观察细胞端粒酶活性,W estern-b lot检测端粒酶蛋白的表达,细胞记数法检测对细胞生长的影响。
- Methods:The full-length gene of NY-ESO-1 was generated by gene splicing method and the recombinant expression vector NY-ESO-1-pET-28a (+) was constructed. E. coli BL21 (DE3) bearing the plasmid was induced with IPTG for protein production. 方法:通过全基因拼接获得NY-ESO-1基因,构建重组表达载体NY-ESO-1-pET28a(+),在大肠杆菌BL21(DE3)中利用IPTG诱导获得表达,利用单克隆抗体进行Western印迹鉴定,通过Ni柱亲和纯化获得纯化蛋白。
- The floor was made of wooden blocks. 地板是用木块拼成的。
- Then the plasmid was transfected into a mouse dendritic cell line, DC2.4, origining from C57BL/ 6 (B6) mouse, by LipofectAMINETM 2000. The expression of mBTIA mRNA and mBTLA-hIg fusion protein were determined by RT-PCR, ELBA and Western blot. 采用RT-PCR、ELISA和Western blot分别检测pmBTLA-hIg质粒转染DC中mBTLA基因mRNA的表达及细胞培养上清中mBTLA-hIg融合蛋白的表达;
- The antique furniture was made in 1700. 这件古老的家具是1700年制造的。
- The dim outline of a house could be made out. 有一所房子的模糊轮廓依稀可辨。
- They made me repeat/I was made to repeat the story. 他们逼我又把那事讲了一遍。
- The legislation was made retrospective. 该项法规具有追溯效力。
- The attack was made under favor of night. 进攻是在黑夜的掩护下进行的。
- The house was made out of stone. 这座房子是用石头建造的。
- Constructed the plasmid "SK-gfp" for transformation. SK-gfp was derived from plasmid pBIN m-gfp5-ER with a length is 4.8 kb which was shorter than pBIN m-gfp5-EK (14.7 kb). These two plasmids were used for the transformation. SK-gfp转化载体的构建 从pBIN m-gfp5-ER(14.;7kb)上酶切出gfp基因，装入SK载体，构建SK-gfp质粒，长度约4
- The plasmid pBV220-Arr. could be expressed in E. 成功构建的重组质粒pBV220-Arr在E.
